TMED2
Gene Ontology Biological Process
Gene Ontology Molecular Function
TMED10
Gene Ontology Biological Process
- COPI coating of Golgi vesicle [TAS]
- COPI-coated vesicle budding [IDA]
- COPII vesicle coating [TAS]
- ER to Golgi vesicle-mediated transport [TAS]
- cargo loading into vesicle [TAS]
- intracellular protein transport [IDA]
- regulated secretory pathway [ISS]
- regulation of beta-amyloid formation [IMP]
- retrograde vesicle-mediated transport, Golgi to ER [ISS]
- vesicle targeting, to, from or within Golgi [ISS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
- COPI-coated vesicle [ISS]
- Golgi apparatus [IDA]
- Golgi membrane [IDA, TAS]
- cis-Golgi network [ISS]
- endoplasmic reticulum [IDA]
- endoplasmic reticulum-Golgi intermediate compartment [IDA]
- extracellular vesicular exosome [IDA]
- gamma-secretase complex [IDA]
- integral component of membrane [ISS]
- plasma membrane [ISS]
- secretory granule membrane [ISS]
- trans-Golgi network transport vesicle [ISS]
- zymogen granule membrane [ISS]
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
OpenCell: Endogenous tagging for the cartography of human cellular organization.
Elucidating the wiring diagram of the human cell is a central goal of the postgenomic era. We combined genome engineering, confocal live-cell imaging, mass spectrometry, and data science to systematically map the localization and interactions of human proteins. Our approach provides a data-driven description of the molecular and spatial networks that organize the proteome. Unsupervised clustering of these networks delineates ... [more]
Throughput
- High Throughput
Additional Notes
- Bait generated from library of CRISPR-edited human embryonic kidney (HEK) 293T cell lines harboring fluorescent tags on individual proteins
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| TMED10 TMED2 | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | High | - | BioGRID | 3373289 | |
| TMED10 TMED2 | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | High | 0.784 | BioGRID | 1175880 | |
| TMED2 TMED10 | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | High | 1 | BioGRID | 3149324 | |
| TMED10 TMED2 | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | High | 0.7985 | BioGRID | 3129947 | |
| TMED2 TMED10 | Co-fractionation Co-fractionation Interaction inferred from the presence of two or more protein subunits in a partially purified protein preparation. If co-fractionation is demonstrated between 3 or more proteins, then add them as a complex. | High | 1 | BioGRID | 1273390 | |
| TMED10 TMED2 | Cross-Linking-MS (XL-MS) Cross-Linking-MS (XL-MS) An interaction is detected between two proteins using chemically reactive or photo-activatable cross-linking reagents that covalently link amino acids in close proximity, followed by mass spectrometry analysis to identify the linked peptides (reviewed in PMID 37406423, 37104977). Experiments may be carried with live cells or cell lysates in which all proteins are expressed at endogenous levels (e.g. PMID 34349018, 35235311) or with recombinant proteins (e.g., PMID 28537071). | High | - | BioGRID | - |
Curated By
- BioGRID