BAIT

PDK1

3'-phosphoinositide-dependent protein kinase 1, 3-PHOSPHOINOSITIDE-DEPENDENT PROTEIN KINASE 1, ATPDK1, T32M21.110, T32M21_110, AT5G04510
3'-phosphoinositide-dependent protein kinase 1
Arabidopsis thaliana (Columbia)
PREY

D6PKL1

AGC1-2, D6 protein kinase like 1, T15N24.60, T15N24_60, AT4G26610
D6 protein kinase like 1
GO Process (0)
GO Function (2)
GO Component (1)

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Arabidopsis thaliana (Columbia)

Reconstituted Complex

An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.

Publication

Structural and functional insights into the regulation of Arabidopsis AGC VIIIa kinases.

Zegzouti H, Li W, Lorenz TC, Xie M, Payne CT, Smith K, Glenny S, Payne GS, Christensen SK

The AGCVIIIa kinases of Arabidopsis are members of the eukaryotic PKA, PKG, and PKC group of regulatory kinases. One AGCVIIIa kinase, PINOID (PID), plays a fundamental role in the asymmetrical localization of membrane proteins during polar auxin transport. The remaining 16 AGCVIIIa genes have not been associated with single mutant phenotypes, suggesting that the corresponding kinases function redundantly. Consistent with ... [more]

J. Biol. Chem. Nov. 17, 2006; 281(46);35520-30 [Pubmed: 16973627]

Throughput

  • Low Throughput

Curated By

  • BioGRID