BAIT

AT4G20380

F9F13.30, F9F13_30, LESION SIMULATING DISEASE, LSD1
zinc finger protein LSD1
Arabidopsis thaliana (Columbia)
PREY

BZO2H1

ATBZIP10, Arabidopsis thaliana basic leucine zipper 10, AT4G02640
basic leucine zipper 10
Arabidopsis thaliana (Columbia)

Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Publication

bZIP10-LSD1 antagonism modulates basal defense and cell death in Arabidopsis following infection.

Kaminaka H, Naeke C, Epple P, Dittgen J, Schuetze K, Chaban C, Holt BF, Merkle T, Schaefer E, Harter K, Dangl JL

Plants use sophisticated strategies to balance responses to oxidative stress. Programmed cell death, including the hypersensitive response (HR) associated with successful pathogen recognition, is one cellular response regulated by reactive oxygen in various cellular contexts. The Arabidopsis basic leucine zipper (bZIP) transcription factor AtbZIP10 shuttles between the nucleus and the cytoplasm and binds consensus G- and C-box DNA sequences. Surprisingly, ... [more]

EMBO J. Sep. 20, 2006; 25(18);4400-11 [Pubmed: 16957775]

Throughput

  • Low Throughput

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
BZO2H1 AT4G20380
PCA
PCA

A Protein-Fragment Complementation Assay (PCA) is a protein-protein interaction assay in which a bait protein is expressed as fusion to one of the either N- or C- terminal peptide fragments of a reporter protein and prey protein is expressed as fusion to the complementary N- or C- terminal fragment of the same reporter protein. Interaction of bait and prey proteins bring together complementary fragments, which can then fold into an active reporter, e.g. the split-ubiquitin assay.

Low-BioGRID
-

Curated By

  • BioGRID