BAIT
VAMP2
SYB2, VAMP-2
vesicle-associated membrane protein 2 (synaptobrevin 2)
GO Process (29)
GO Function (9)
GO Component (25)
Gene Ontology Biological Process
- Golgi to plasma membrane protein transport [ISS]
- calcium ion-dependent exocytosis [ISS]
- cellular protein metabolic process [TAS]
- cellular response to insulin stimulus [ISS]
- energy reserve metabolic process [TAS]
- eosinophil degranulation [IMP]
- exocytosis [IBA, TAS]
- glutamate secretion [TAS]
- long-term synaptic potentiation [ISS]
- membrane fusion [ISS]
- membrane organization [TAS]
- mucus secretion [IMP]
- natural killer cell degranulation [IMP]
- neurotransmitter secretion [TAS]
- neutrophil degranulation [IMP]
- positive regulation of intracellular protein transport [ISS]
- post-Golgi vesicle-mediated transport [TAS]
- protein complex assembly [ISS]
- protein transport [ISS]
- regulation of exocytosis [ISS]
- regulation of histamine secretion by mast cell [IMP]
- regulation of insulin secretion [TAS]
- regulation of vesicle-mediated transport [ISS]
- response to glucose [ISS]
- small molecule metabolic process [TAS]
- synaptic transmission [TAS]
- synaptic vesicle exocytosis [ISS, TAS]
- vesicle fusion [IBA]
- vesicle-mediated transport [ISS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
- SNARE complex [IDA, TAS]
- clathrin-coated vesicle [IDA]
- clathrin-sculpted gamma-aminobutyric acid transport vesicle membrane [TAS]
- clathrin-sculpted glutamate transport vesicle membrane [TAS]
- clathrin-sculpted monoamine transport vesicle membrane [TAS]
- cytoplasmic vesicle [ISS]
- endocytic vesicle membrane [TAS]
- extracellular vesicular exosome [IDA]
- integral component of plasma membrane [TAS]
- intracellular membrane-bounded organelle [ISS]
- membrane [IDA]
- neuron projection [ISS]
- neuron projection terminus [ISS]
- perinuclear region of cytoplasm [ISS]
- plasma membrane [ISS, TAS]
- secretory granule [ISS]
- secretory granule membrane [IDA, TAS]
- synapse [ISS]
- synaptic vesicle [IBA, ISS, TAS]
- synaptic vesicle membrane [ISS]
- synaptobrevin 2-SNAP-25-syntaxin-1a complex [ISS]
- synaptobrevin 2-SNAP-25-syntaxin-1a-complexin I complex [ISS]
- synaptobrevin 2-SNAP-25-syntaxin-1a-complexin II complex [ISS]
- trans-Golgi network [ISS]
- zymogen granule membrane [ISS]
Homo sapiens
PREY
NSF
SKD2
N-ethylmaleimide-sensitive factor
GO Process (10)
GO Function (7)
GO Component (8)
Gene Ontology Biological Process
- ATP catabolic process [ISS]
- Golgi to plasma membrane protein transport [IBA]
- Golgi vesicle docking [IBA]
- exocytosis [TAS]
- intra-Golgi vesicle-mediated transport [IBA]
- plasma membrane fusion [TAS]
- positive regulation of receptor recycling [IDA]
- regulation of exocytosis [ISS]
- synaptic transmission [TAS]
- vesicle-mediated transport [ISS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
OpenCell: Endogenous tagging for the cartography of human cellular organization.
Elucidating the wiring diagram of the human cell is a central goal of the postgenomic era. We combined genome engineering, confocal live-cell imaging, mass spectrometry, and data science to systematically map the localization and interactions of human proteins. Our approach provides a data-driven description of the molecular and spatial networks that organize the proteome. Unsupervised clustering of these networks delineates ... [more]
Science Dec. 11, 2021; 375(6585);eabi6983 [Pubmed: 35271311]
Throughput
- High Throughput
Additional Notes
- Bait generated from library of CRISPR-edited human embryonic kidney (HEK) 293T cell lines harboring fluorescent tags on individual proteins
Curated By
- BioGRID