BAIT
VCL
CMD1W, CMH15, HEL114, MV, MVCL, RP11-178G16.3
vinculin
GO Process (15)
GO Function (6)
GO Component (12)
Gene Ontology Biological Process
- adherens junction assembly [IMP]
- apical junction assembly [IMP]
- blood coagulation [TAS]
- cell adhesion [TAS]
- cell-matrix adhesion [TAS]
- cellular component movement [TAS]
- epithelial cell-cell adhesion [IMP]
- lamellipodium assembly [ISS]
- morphogenesis of an epithelium [IMP]
- muscle contraction [TAS]
- negative regulation of cell migration [TAS]
- platelet activation [TAS]
- platelet aggregation [IMP]
- platelet degranulation [TAS]
- protein localization to cell surface [IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
PREY
RCC2
TD-60
regulator of chromosome condensation 2
GO Process (10)
GO Function (4)
GO Component (4)
Gene Ontology Biological Process
- chromosome passenger complex localization to kinetochore [IMP]
- focal adhesion assembly [IDA]
- integrin-mediated signaling pathway [IDA]
- mitotic cell cycle [TAS]
- negative regulation of GTPase activity [ISS]
- negative regulation of focal adhesion assembly [ISS]
- negative regulation of substrate adhesion-dependent cell spreading [ISS]
- positive regulation of G2/M transition of mitotic cell cycle [IMP]
- positive regulation of attachment of spindle microtubules to kinetochore [IMP]
- regulation of cell migration [ISS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
OpenCell: Endogenous tagging for the cartography of human cellular organization.
Elucidating the wiring diagram of the human cell is a central goal of the postgenomic era. We combined genome engineering, confocal live-cell imaging, mass spectrometry, and data science to systematically map the localization and interactions of human proteins. Our approach provides a data-driven description of the molecular and spatial networks that organize the proteome. Unsupervised clustering of these networks delineates ... [more]
Science Dec. 11, 2021; 375(6585);eabi6983 [Pubmed: 35271311]
Throughput
- High Throughput
Additional Notes
- Bait generated from library of CRISPR-edited human embryonic kidney (HEK) 293T cell lines harboring fluorescent tags on individual proteins
Curated By
- BioGRID