BAIT

BZO2H1

ATBZIP10, Arabidopsis thaliana basic leucine zipper 10, AT4G02640
basic leucine zipper 10
Arabidopsis thaliana (Columbia)
PREY

BZIP9

ARABIDOPSIS THALIANA BASIC LEUCINE ZIPPER 9, ATBZIP9, BASIC LEUCINE ZIPPER O2 HOMOLOG 2, BZIP PROTEIN BZO2H2, BZO2H2, F6A4.10, F6A4_10, basic leucine zipper 9, AT5G24800
basic leucine zipper 9
GO Process (1)
GO Function (5)
GO Component (1)
Arabidopsis thaliana (Columbia)

Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Publication

Two-hybrid protein-protein interaction analysis in Arabidopsis protoplasts: establishment of a heterodimerization map of group C and group S bZIP transcription factors.

Ehlert A, Weltmeier F, Wang X, Mayer CS, Smeekens S, Vicente-Carbajosa J, Droege-Laser W

In vivo protein-protein interactions are frequently studied by means of yeast two-hybrid analysis. However, interactions detected in yeast might differ considerably in the plant system. Based on GAL4 DNA-binding (BD) and activation domains (AD) we established an Arabidopsis protoplast two-hybrid (P2H) system. The use of Gateway-compatible vectors enables the high-throughput screening of protein-protein interactions in plant cells. The efficiency of ... [more]

Plant J. Jun. 01, 2006; 46(5);890-900 [Pubmed: 16709202]

Throughput

  • Low Throughput

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
BZIP9 BZO2H1
Two-hybrid
Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Low-BioGRID
-

Curated By

  • BioGRID