BAIT
YWHAE
14-3-3E, HEL2, KCIP-1, MDCR, MDS
tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, epsilon
GO Process (18)
GO Function (10)
GO Component (5)
Gene Ontology Biological Process
- G2/M transition of mitotic cell cycle [TAS]
- apoptotic process [TAS]
- apoptotic signaling pathway [TAS]
- hippo signaling [TAS]
- intracellular signal transduction [TAS]
- intrinsic apoptotic signaling pathway [TAS]
- membrane organization [TAS]
- membrane repolarization during cardiac muscle cell action potential [IC]
- mitotic cell cycle [TAS]
- negative regulation of peptidyl-serine dephosphorylation [IDA]
- neurotrophin TRK receptor signaling pathway [TAS]
- positive regulation of protein insertion into mitochondrial membrane involved in apoptotic signaling pathway [TAS]
- regulation of cysteine-type endopeptidase activity involved in apoptotic process [TAS]
- regulation of heart rate by cardiac conduction [IC]
- regulation of heart rate by hormone [NAS]
- regulation of membrane repolarization [IDA]
- regulation of potassium ion transmembrane transporter activity [IDA]
- substantia nigra development [IEP]
Gene Ontology Molecular Function- MHC class II protein complex binding [IDA]
- enzyme binding [IPI]
- histone deacetylase binding [IPI]
- ion channel binding [IPI]
- phosphoprotein binding [IPI]
- phosphoserine binding [IPI]
- poly(A) RNA binding [IDA]
- potassium channel regulator activity [IDA]
- protein binding [IPI]
- protein heterodimerization activity [IPI]
- MHC class II protein complex binding [IDA]
- enzyme binding [IPI]
- histone deacetylase binding [IPI]
- ion channel binding [IPI]
- phosphoprotein binding [IPI]
- phosphoserine binding [IPI]
- poly(A) RNA binding [IDA]
- potassium channel regulator activity [IDA]
- protein binding [IPI]
- protein heterodimerization activity [IPI]
Gene Ontology Cellular Component
Homo sapiens
PREY
PRKD1
PKC-MU, PKCM, PKD, PRKCM
protein kinase D1
GO Process (33)
GO Function (3)
GO Component (5)
Gene Ontology Biological Process
- Golgi organization [IMP]
- Golgi vesicle transport [ISS]
- cell proliferation [TAS]
- cellular response to oxidative stress [IDA]
- cellular response to vascular endothelial growth factor stimulus [IGI, IMP]
- integrin-mediated signaling pathway [TAS]
- intracellular signal transduction [IMP]
- negative regulation of cell death [IMP]
- negative regulation of endocytosis [TAS]
- peptidyl-serine phosphorylation [IDA]
- positive regulation of CREB transcription factor activity [IGI]
- positive regulation of I-kappaB kinase/NF-kappaB signaling [IMP]
- positive regulation of NF-kappaB transcription factor activity [IMP]
- positive regulation of angiogenesis [IGI, IMP]
- positive regulation of blood vessel endothelial cell migration [IGI, IMP]
- positive regulation of endothelial cell chemotaxis [IMP]
- positive regulation of endothelial cell chemotaxis by VEGF-activated vascular endothelial growth factor receptor signaling pathway [IGI, IMP]
- positive regulation of endothelial cell migration [IMP]
- positive regulation of endothelial cell proliferation [IGI]
- positive regulation of histone deacetylase activity [IGI]
- positive regulation of neuron projection development [IMP]
- positive regulation of osteoblast differentiation [ISS]
- positive regulation of peptidyl-serine phosphorylation [IGI]
- positive regulation of transcription from RNA polymerase II promoter [IMP]
- protein autophosphorylation [TAS]
- protein kinase D signaling [IGI]
- regulation of integrin-mediated signaling pathway [TAS]
- regulation of keratinocyte proliferation [ISS]
- signal transduction [TAS]
- small molecule metabolic process [TAS]
- sphingolipid biosynthetic process [TAS]
- sphingolipid metabolic process [TAS]
- vascular endothelial growth factor receptor signaling pathway [IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
OpenCell: Endogenous tagging for the cartography of human cellular organization.
Elucidating the wiring diagram of the human cell is a central goal of the postgenomic era. We combined genome engineering, confocal live-cell imaging, mass spectrometry, and data science to systematically map the localization and interactions of human proteins. Our approach provides a data-driven description of the molecular and spatial networks that organize the proteome. Unsupervised clustering of these networks delineates ... [more]
Science Dec. 11, 2021; 375(6585);eabi6983 [Pubmed: 35271311]
Throughput
- High Throughput
Additional Notes
- Bait generated from library of CRISPR-edited human embryonic kidney (HEK) 293T cell lines harboring fluorescent tags on individual proteins
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| PRKD1 YWHAE | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | High | - | BioGRID | - |
Curated By
- BioGRID