BAIT
CCDC47
MSTP041, GK001
coiled-coil domain containing 47
GO Process (1)
GO Function (2)
GO Component (1)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Homo sapiens
PREY
WFS1
CTRCT41, WFRS, WFS, WFSL
Wolfram syndrome 1 (wolframin)
GO Process (27)
GO Function (4)
GO Component (4)
Gene Ontology Biological Process
- ER overload response [IC, TAS]
- ER-associated ubiquitin-dependent protein catabolic process [ISS]
- activation of signaling protein activity involved in unfolded protein response [TAS]
- calcium ion homeostasis [IDA]
- cellular protein metabolic process [TAS]
- endoplasmic reticulum calcium ion homeostasis [IDA]
- endoplasmic reticulum unfolded protein response [ISS, TAS]
- glucose homeostasis [IMP]
- kidney development [IMP]
- negative regulation of endoplasmic reticulum stress-induced intrinsic apoptotic signaling pathway [ISS]
- negative regulation of neuron apoptotic process [IMP]
- negative regulation of programmed cell death [IMP]
- negative regulation of sequence-specific DNA binding transcription factor activity [ISS]
- negative regulation of type B pancreatic cell apoptotic process [IMP]
- neurological system process [IMP]
- polyubiquitinated misfolded protein transport [ISS]
- positive regulation of calcium ion transport [IDA]
- positive regulation of growth [ISS]
- positive regulation of protein metabolic process [IDA]
- positive regulation of protein ubiquitination [ISS]
- positive regulation of proteolysis [ISS]
- protein maturation by protein folding [IC]
- protein stabilization [ISS]
- renal water homeostasis [IMP]
- response to endoplasmic reticulum stress [IDA]
- sensory perception of sound [IMP]
- visual perception [IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
OpenCell: Endogenous tagging for the cartography of human cellular organization.
Elucidating the wiring diagram of the human cell is a central goal of the postgenomic era. We combined genome engineering, confocal live-cell imaging, mass spectrometry, and data science to systematically map the localization and interactions of human proteins. Our approach provides a data-driven description of the molecular and spatial networks that organize the proteome. Unsupervised clustering of these networks delineates ... [more]
Science Dec. 11, 2021; 375(6585);eabi6983 [Pubmed: 35271311]
Throughput
- High Throughput
Additional Notes
- Bait generated from library of CRISPR-edited human embryonic kidney (HEK) 293T cell lines harboring fluorescent tags on individual proteins
Curated By
- BioGRID