BAIT

ICE1

A. THALIANA INDUCER OF CBP EXPRESSION 1, ATICE1, INDUCER OF CBF EXPRESSION 1, SCREAM, SCRM, AT3G26744
transcription factor ICE1
GO Process (4)
GO Function (2)
GO Component (1)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Arabidopsis thaliana (Columbia)
PREY

UBQ1

EARLY-RESPONSIVE TO DEHYDRATION 16, EMB2167, EMBRYO DEFECTIVE 2167, ERD16, F3C22.8, HAP4, HAPLESS 4, ubiquitin extension protein 1, AT3G52590
ubiquitin extension protein 1
GO Process (2)
GO Function (1)
GO Component (0)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Arabidopsis thaliana (Columbia)

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

The negative regulator of plant cold responses, HOS1, is a RING E3 ligase that mediates the ubiquitination and degradation of ICE1.

Dong CH, Agarwal M, Zhang Y, Xie Q, Zhu JK

Plant responses to cold stress are mediated by a transcriptional cascade, in which the transcription factor ICE1 and possibly related proteins activate the expression of C-repeat (CRT)-binding factors (CBFs), leading to the transcription of downstream effector genes. The variant RING finger protein high expression of osmotically responsive gene (HOS)1 was identified genetically as a negative regulator of cold responses. We ... [more]

Proc. Natl. Acad. Sci. U.S.A. May. 23, 2006; 103(21);8281-6 [Pubmed: 16702557 ]

Throughput

  • Low Throughput

Curated By

  • BioGRID