BAIT

RPO21

RPB1, RPB220, SUA8, DNA-directed RNA polymerase II core subunit RPO21, B220, L000001744, YDL140C
RNA polymerase II largest subunit B220; part of central core; phosphorylation of C-terminal heptapeptide repeat domain regulates association with transcription and splicing factors; similar to bacterial beta-prime
GO Process (2)
GO Function (2)
GO Component (3)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Saccharomyces cerevisiae (S288c)
PREY

MOG1

L000004615, YJR074W
Conserved nuclear protein that interacts with GTP-Gsp1p; stimulates nucleotide release from Gsp1p; involved in nuclear protein import; nucleotide release is inhibited by Yrb1p
GO Process (1)
GO Function (1)
GO Component (1)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Saccharomyces cerevisiae (S288c)

Negative Genetic

Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.

Publication

High-throughput genetic and gene expression analysis of the RNAPII-CTD reveals unexpected connections to SRB10/CDK8.

Aristizabal MJ, Negri GL, Benschop JJ, Holstege FC, Krogan NJ, Kobor MS

The C-terminal domain (CTD) of RNA polymerase II (RNAPII) is composed of heptapeptide repeats, which play a key regulatory role in gene expression. Using genetic interaction, chromatin immunoprecipitation followed by microarrays (ChIP-on-chip) and mRNA expression analysis, we found that truncating the CTD resulted in distinct changes to cellular function. Truncating the CTD altered RNAPII occupancy, leading to not only decreases, ... [more]

PLoS Genet. Aug. 01, 2013; 9(8);e1003758 [Pubmed: 24009531]

Quantitative Score

  • -15.072645 [Confidence Score]

Throughput

  • High Throughput

Ontology Terms

  • colony size (APO:0000063)

Additional Notes

  • RPB1 - CTD11
  • RPB1 - CTD13
  • RPB1 - CTD20
  • significance threshold S<=-2.5, S>=2

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
MOG1 RPO21
Negative Genetic
Negative Genetic

Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.

High-3.2878BioGRID
308385

Curated By

  • BioGRID