An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

A human DNA polymerase eta complex containing Rad18, Rad6 and Rev1; proteomic analysis and targeting of the complex to the chromatin-bound fraction of cells undergoing replication fork arrest.

Yuasa MS, Masutani C, Hirano A, Cohn MA, Yamaizumi M, Nakatani Y, Hanaoka F

DNA polymerase eta (Poleta) is responsible for efficient translesion synthesis (TLS) past cis-syn cyclobutane thymine dimers (TT dimers), the major DNA lesions induced by UV irradiation. Loss of human Poleta leads to xeroderma pigmentosum variant syndrome, clearly indicating that Poleta plays a vital role in preventing skin cancer caused by exposure to sunlight. To further examine Poleta functions and the ... [more]

Genes Cells Jul. 01, 2006; 11(7);731-44 [Pubmed: 16824193]

Throughput

Low Throughput

Curated By

BioGRID

Interaction Summary

POLH

Gene Ontology Biological Process

Gene Ontology Molecular Function

DNA-directed DNA polymerase activity [TAS]protein binding [IPI]

Gene Ontology Cellular Component

TUBA1B

Gene Ontology Biological Process

Gene Ontology Molecular Function

double-stranded RNA binding [IDA]protein binding [IPI]structural molecule activity [TAS]ubiquitin protein ligase binding [IPI]

Gene Ontology Cellular Component

Affinity Capture-MS

Publication

A human DNA polymerase eta complex containing Rad18, Rad6 and Rev1; proteomic analysis and targeting of the complex to the chromatin-bound fraction of cells undergoing replication fork arrest.

Throughput

Curated By

DNA-directed DNA polymerase activity [TAS]
protein binding [IPI]

double-stranded RNA binding [IDA]
protein binding [IPI]
structural molecule activity [TAS]
ubiquitin protein ligase binding [IPI]