Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

SNX27-driven membrane localisation of OTULIN antagonises linear ubiquitination and NF-?B signalling activation.

Shi R, Shi X, Qin D, Tang S, Vermeulen M, Zhang X

Linear ubiquitination is a novel type of ubiquitination that plays important physiological roles in signalling pathways such as tumour necrosis factor (TNF) signalling. However, little is known about the regulatory mechanisms of linear ubiquitination, except the well-described enzymatic regulators E3 ligase linear ubiquitin chain assembly complex (LUBAC) and deubiquitinase OTULIN.Previously, we identified SNX27, a member of the sorting nexin family ... [more]

Cell Biosci Jul. 27, 2021; 11(1);146 [Pubmed: 34315543]

Throughput

  • High Throughput

Additional Notes

  • Affinity Capture-MS was carried out to identify high confidence protein-protein interactors with a fold change of >2 over control and an adjusted p<0.05

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
SNX27 DGKZ
Affinity Capture-Western
Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Low-BioGRID
-

Curated By

  • BioGRID