Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

SNX27-driven membrane localisation of OTULIN antagonises linear ubiquitination and NF-?B signalling activation.

Shi R, Shi X, Qin D, Tang S, Vermeulen M, Zhang X

Linear ubiquitination is a novel type of ubiquitination that plays important physiological roles in signalling pathways such as tumour necrosis factor (TNF) signalling. However, little is known about the regulatory mechanisms of linear ubiquitination, except the well-described enzymatic regulators E3 ligase linear ubiquitin chain assembly complex (LUBAC) and deubiquitinase OTULIN.Previously, we identified SNX27, a member of the sorting nexin family ... [more]

Cell Biosci Jul. 27, 2021; 11(1);146 [Pubmed: 34315543]

Throughput

  • High Throughput

Additional Notes

  • Affinity Capture-MS was carried out to identify high confidence protein-protein interactors with a fold change of >2 over control and an adjusted p<0.05

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
PHLPP1 SNX27
Affinity Capture-MS
Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

High1.92BioGRID
424281
PHLPP1 SNX27
Proximity Label-MS
Proximity Label-MS

An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.

High-BioGRID
3388400

Curated By

  • BioGRID