An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

The histone variant mH2A1.1 interferes with transcription by down-regulating PARP-1 enzymatic activity.

Ouararhni K, Hadj-Slimane R, Ait-Si-Ali S, Robin P, Mietton F, Harel-Bellan A, Dimitrov S, Hamiche A

The histone variant mH2A is believed to be involved in transcriptional repression, but how it exerts its function remains elusive. By using chromatin immunoprecipitation and tandem affinity immunopurification of the mH2A1.1 nucleosome complex, we identified numerous genes with promoters containing mH2A1.1 nucleosomes. In particular, the promoters of the inducible Hsp70.1 and Hsp70.2 genes, but not that of the constitutively expressed ... [more]

Genes Dev. Dec. 01, 2006; 20(23);3324-36 [Pubmed: 17158748]

Throughput

Low Throughput

Curated By

BioGRID

Interaction Summary

H2AFY

Gene Ontology Biological Process

Gene Ontology Molecular Function

DNA binding [NAS]chromatin DNA binding [IDA]double-stranded methylated DNA binding [IDA]protein binding [IPI]protein kinase binding [IPI]protein serine/threonine kinase inhibitor activity [IMP]rDNA binding [IDA]transcription regulatory region DNA binding [IDA]

Gene Ontology Cellular Component

HSPA4