An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.

Publication

RNF43 inhibits WNT5A-driven signaling and suppresses melanoma invasion and resistance to the targeted therapy.

Radaszkiewicz T, Noskova M, Goemoeryova K, Vondalova Blanarova O, Radaszkiewicz KA, Pickova M, Vichova R, Gybel T, Kaiser K, Demkova L, Kucerova L, Barta T, Potesil D, Zdrahal Z, Soucek K, Bryja V

RNF43 is an E3 ubiquitin ligase and known negative regulator of WNT/?-catenin signaling. We demonstrate that RNF43 is also a regulator of noncanonical WNT5A-induced signaling in human cells. Analysis of the RNF43 interactome using BioID and immunoprecipitation showed that RNF43 can interact with the core receptor complex components dedicated to the noncanonical Wnt pathway such as ROR1, ROR2, VANGL1, and ... [more]

Elife Dec. 27, 2020; 10(); [Pubmed: 34702444]

Throughput

Low Throughput

Additional Notes

BioID

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
RNF43 DDX5	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Fang L (2022)	Low	-	BioGRID	-

Curated By

BioGRID

Interaction Summary

RNF43

Gene Ontology Biological Process

Gene Ontology Molecular Function

frizzled binding [IPI]protein binding [IPI]ubiquitin-protein transferase activity [IDA]

Gene Ontology Cellular Component

DDX5

Gene Ontology Biological Process

Gene Ontology Molecular Function

RNA helicase activity [IMP, NAS]androgen receptor binding [IDA]estrogen receptor binding [IDA]poly(A) RNA binding [IDA]pre-mRNA binding [IDA]protein binding [IPI]transcription coactivator activity [IDA]