An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Degradation of survivin by the X-linked inhibitor of apoptosis (XIAP)-XAF1 complex.

Arora V, Cheung HH, Plenchette S, Micali OC, Liston P, Korneluk RG

X-linked inhibitor of apoptosis (XIAP)-associated factor 1 (XAF1) is a putative tumor suppressor in which expression is significantly reduced in human cancer cell lines and primary tumors. The proapoptotic effects of XAF1 have been attributed to both caspase-dependent and -independent means. In particular, XAF1 reverses the anti-caspase activity of XIAP, a physiological inhibitor of apoptosis. We further investigated the function ... [more]

J. Biol. Chem. Sep. 07, 2007; 282(36);26202-9 [Pubmed: 17613533]

Throughput

Low Throughput

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
BIRC7 XAF1	Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.	Luck K (2020)	High	-	BioGRID	2706531

Curated By

BioGRID

Interaction Summary

BIRC7

Gene Ontology Biological Process

Gene Ontology Molecular Function

cysteine-type endopeptidase inhibitor activity [TAS]cysteine-type endopeptidase inhibitor activity involved in apoptotic process [IBA]enzyme binding [NAS]protein binding [IPI]ubiquitin-protein transferase activity [IDA]

Gene Ontology Cellular Component

XAF1