Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

AMBRA1 regulates mitophagy by interacting with ATAD3A and promoting PINK1 stability.

Di Rienzo M, Romagnoli A, Ciccosanti F, Refolo G, Consalvi V, Arena G, Valente EM, Piacentini M, Fimia GM

PINK1 accumulation at the outer mitochondrial membrane (OMM) is a key event required to signal depolarized mitochondria to the autophagy machinery. How this early step is, in turn, modulated by autophagy proteins remains less characterized. Here, we show that, upon mitochondrial depolarization, the proautophagic protein AMBRA1 is recruited to the OMM and interacts with PINK1 and ATAD3A, a transmembrane protein ... [more]

Autophagy Nov. 19, 2021; ();1-11 [Pubmed: 34798798]

Throughput

  • Low Throughput

Curated By

  • BioGRID