Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Loss of TRIM31 promotes breast cancer progression through regulating K48- and K63-linked ubiquitination of p53.

Guo Y, Li Q, Zhao G, Zhang J, Yuan H, Feng T, Ou D, Gu R, Li S, Li K, Lin P

Breast cancer is the most common cancer in the world. Relapse and metastasis are important factors endangering the life of breast cancer patients, but the mechanism is still unclear. The stabilization of p53 is essential for preventing carcinogenesis, and ubiquitination is one of the main ways to regulate the stability of p53. Tripartite motif-containing 31 (TRIM31) is a new member ... [more]

Cell Death Dis Dec. 14, 2020; 12(10);945 [Pubmed: 34650049]

Throughput

  • Low Throughput

Curated By

  • BioGRID