BAIT

CLPP

DFNB81, PRLTS3
caseinolytic mitochondrial matrix peptidase proteolytic subunit
GO Process (1)
GO Function (3)
GO Component (3)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Homo sapiens
PREY

TMEM33

1600019D15Rik, SHINC3
transmembrane protein 33
GO Process (0)
GO Function (0)
GO Component (2)

Gene Ontology Cellular Component

Homo sapiens

Proximity Label-MS

An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.

Publication

Variability in Streptavidin-Sepharose Matrix Quality Can Significantly Affect Proximity-Dependent Biotinylation (BioID) Data.

St-Germain JR, Samavarchi Tehrani P, Wong C, Larsen B, Gingras AC, Raught B

Due to their ease of use and high binding affinity, streptavidin-based purification tools have become widely used for isolating biotinylated compounds from complex mixtures. We and others routinely use streptavidin-sepharose matrices to isolate biotinylated polypeptides generated in proximity-dependent biotinylation approaches, such as BioID or APEX. However, we noted sporadic, substantial variation in the quality of BioID experiments performed in the ... [more]

J Proteome Res Dec. 07, 2019; 19(8);3554-3561 [Pubmed: 32628020]

Throughput

  • High Throughput

Additional Notes

  • BFDR less or equal to 0.01
  • BioID

Curated By

  • BioGRID