An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.

Publication

Variability in Streptavidin-Sepharose Matrix Quality Can Significantly Affect Proximity-Dependent Biotinylation (BioID) Data.

St-Germain JR, Samavarchi Tehrani P, Wong C, Larsen B, Gingras AC, Raught B

Due to their ease of use and high binding affinity, streptavidin-based purification tools have become widely used for isolating biotinylated compounds from complex mixtures. We and others routinely use streptavidin-sepharose matrices to isolate biotinylated polypeptides generated in proximity-dependent biotinylation approaches, such as BioID or APEX. However, we noted sporadic, substantial variation in the quality of BioID experiments performed in the ... [more]

J Proteome Res Dec. 07, 2019; 19(8);3554-3561 [Pubmed: 32628020]

Throughput

High Throughput

Additional Notes

BFDR less or equal to 0.01
BioID

Curated By

BioGRID

Interaction Summary

CLPP

Gene Ontology Biological Process

Gene Ontology Molecular Function

peptidase activity [TAS]protein binding [IPI]serine-type endopeptidase activity [IDA]

Gene Ontology Cellular Component

CDIPT