CLPP
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
RHOG
Gene Ontology Biological Process
- GTP catabolic process [IDA, TAS]
- Rac protein signal transduction [IDA]
- Rho protein signal transduction [IDA]
- actin cytoskeleton organization [IDA]
- activation of Rac GTPase activity [IMP]
- axon guidance [TAS]
- blood coagulation [TAS]
- cell chemotaxis [IMP]
- platelet activation [TAS]
- positive regulation of cell proliferation [TAS]
- positive regulation of establishment of protein localization to plasma membrane [IMP]
- positive regulation of transcription, DNA-templated [IDA]
- regulation of small GTPase mediated signal transduction [TAS]
- small GTPase mediated signal transduction [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Proximity Label-MS
An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.
Publication
Variability in Streptavidin-Sepharose Matrix Quality Can Significantly Affect Proximity-Dependent Biotinylation (BioID) Data.
Due to their ease of use and high binding affinity, streptavidin-based purification tools have become widely used for isolating biotinylated compounds from complex mixtures. We and others routinely use streptavidin-sepharose matrices to isolate biotinylated polypeptides generated in proximity-dependent biotinylation approaches, such as BioID or APEX. However, we noted sporadic, substantial variation in the quality of BioID experiments performed in the ... [more]
Throughput
- High Throughput
Additional Notes
- BFDR less or equal to 0.01
- BioID
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| RHOG CLPP | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | High | - | BioGRID | 3336957 |
Curated By
- BioGRID