Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Publication

Pellino-2 in nonimmune cells: novel interaction partners and intracellular localization.

Cristea I, Bruland O, Aukrust I, Rodahl E, Bredrup C

Pellino-2 is an E3 ubiquitin ligase that mediates intracellular signaling in innate immune pathways. Most studies of endogenous Pellino-2 have been performed in macrophages, but none in nonimmune cells. Using yeast two-hybrid screening and co-immunoprecipitation, we identified six novel interaction partners of Pellino-2, with various localizations: insulin receptor substrate 1, NIMA-related kinase 9, tumor necrosis factor receptor-associated factor 7, cyclin-F, ... [more]

FEBS Lett Dec. 01, 2020; 595(23);2909-2921 [Pubmed: 34674267]

Throughput

  • Low Throughput

Curated By

  • BioGRID