BAIT

CTF4

CHL15, POB1, chromatin-binding protein CTF4, L000000326, YPR135W
Chromatin-associated protein; required for sister chromatid cohesion; interacts with DNA polymerase alpha (Pol1p) and may link DNA synthesis to sister chromatid cohesion
Saccharomyces cerevisiae (S288c)
PREY

UBC1

E2 ubiquitin-conjugating protein UBC1, L000002406, YDR177W
Ubiquitin-conjugating enzyme; key E2 partner with Ubc4p for the anaphase-promoting complex (APC); mediates selective degradation of short-lived and abnormal proteins; plays a role in vesicle biogenesis and ER-associated protein degradation (ERAD); component of the cellular stress response; protein abundance increases in response to DNA replication stress key E2 partner with Ubc4p for the anaphase-promoting complex (APC)
GO Process (3)
GO Function (1)
GO Component (1)
Saccharomyces cerevisiae (S288c)

Synthetic Lethality

A genetic interaction is inferred when mutations or deletions in separate genes, each of which alone causes a minimal phenotype, result in lethality when combined in the same cell under a given condition.

Publication

Ubc1 turnover contributes to the spindle assembly checkpoint in Saccharomyces cerevisiae.

Arsenault HE, Ghizzoni JM, Leech CM, Diers AR, Gesta S, Vishnudas VK, Narain NR, Sarangarajan R, Benanti JA

The spindle assembly checkpoint protects the integrity of the genome by ensuring that chromosomes are properly attached to the mitotic spindle before they are segregated during anaphase. Activation of the spindle checkpoint results in inhibition of the Anaphase-Promoting Complex (APC), an E3 ubiquitin ligase that triggers the metaphase-anaphase transition. Here, we show that levels of Ubc1, an E2 enzyme that ... [more]

G3 (Bethesda) Dec. 08, 2020; 11(12); [Pubmed: 34586382]

Throughput

  • Low Throughput

Ontology Terms

  • inviable (APO:0000112)

Additional Notes

  • Ubc1 shut-off was lethal in a ctf4 deletion strain

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
UBC1 CTF4
Negative Genetic
Negative Genetic

Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.

High-0.4349BioGRID
1969193

Curated By

  • BioGRID