BAIT

RAP1GAP

RAP1GA1, RAP1GAP1, RAP1GAPII, RAPGAP, RP11-63N8.1
RAP1 GTPase activating protein
Homo sapiens

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

The degradation of Rap1GAP via E6AP-mediated ubiquitin-proteasome pathway is associated with HPV16/18-infection in cervical cancer cells.

Wang Y, Xie Y, Sun B, Guo Y, Song L, Mohammednur DE, Zhao C

Cervical cancers are closely associated with persistent high-risk human papillomaviruses (HR HPV) infection. The main mechanism involves the targeting of tumor suppressors, such as p53 and pRB, for degradation by HR HPV-encoded oncoproteins, thereby leading to tumorigenesis. Rap1GAP, a tumor suppressor gene, is down-regulated in many cancers. Previous studies have revealed that down-regulation of Rap1GAP is correlated with HPV16/18 infection ... [more]

Infect Agent Cancer Dec. 24, 2021; 16(1);71 [Pubmed: 34952616]

Throughput

  • Low Throughput

Curated By

  • BioGRID