Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Oncogenic Activity of Glucocorticoid Receptor ? Is Controlled by Ubiquitination-Dependent Interaction with USP49 in Glioblastoma Cells.

Hu Y, Jiang Y, Zhang Z, Wang J, Zhang B, Gong L, Ji L, Pu Z, Yang X, Zou J, Yin Y

Previous studies have demonstrated that glucocorticoid receptor ? (GR?) functions as an oncoprotein, regulating the malignant phenotypes and stem-like cell maintaining in human glioblastoma (GBM). Of the glucocorticoid receptor (GR) isoforms, GR? and GR? are highly homologous, though the mechanism underlying the distinct functions of these two isoforms in GBM has not been clarified. Here by establishing a carboxyl-terminal (COOH-terminal) ... [more]

Mol Cancer Res Dec. 01, 2021; 20(1);92-101 [Pubmed: 34610959]

Throughput

  • Low Throughput

Additional Notes

  • GR beta isoform

Curated By

  • BioGRID