BAIT
USP8
AI574262, AW557536, Ubpy, mKIAA0055, RP23-130L11.1
ubiquitin specific peptidase 8
GO Process (9)
GO Function (4)
GO Component (10)
Gene Ontology Biological Process
- Ras protein signal transduction [IPI]
- endosome organization [IMP, ISO]
- mitotic cytokinesis [ISO]
- proteasome-mediated ubiquitin-dependent protein catabolic process [IBA]
- protein K48-linked deubiquitination [ISO]
- protein K63-linked deubiquitination [ISO]
- protein deubiquitination [IDA, IMP, ISO]
- proteolysis [ISO]
- regulation of proteasomal protein catabolic process [IBA]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Mus musculus
PREY
YWHAZ
14-3-3-zeta, HEL-S-3, HEL4, KCIP-1, YWHAD
tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, zeta
GO Process (13)
GO Function (5)
GO Component (9)
Gene Ontology Biological Process
- Golgi reassembly [IMP]
- RNA metabolic process [TAS]
- apoptotic process [TAS]
- blood coagulation [TAS]
- establishment of Golgi localization [IMP]
- gene expression [TAS]
- intrinsic apoptotic signaling pathway [TAS]
- mRNA metabolic process [TAS]
- membrane organization [TAS]
- negative regulation of apoptotic process [TAS]
- platelet activation [TAS]
- positive regulation of protein insertion into mitochondrial membrane involved in apoptotic signaling pathway [TAS]
- signal transduction [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
14-3-3-dependent inhibition of the deubiquitinating activity of UBPY and its cancellation in the M phase.
The deubiquitinating enzyme UBPY, also known as USP8, regulates cargo sorting and membrane traffic at early endosomes. Here we demonstrate the regulatory mechanism of the UBPY catalytic activity. We identified 14-3-3 epsilon, gamma, and zeta as UBPY-binding proteins using co-immunoprecipitation followed by mass spectrometric analysis. The 14-3-3 binding of UBPY was inhibited by mutating the consensus 14-3-3-binding motif RSYS(680)SP, by ... [more]
Exp. Cell Res. Oct. 01, 2007; 313(16);3624-34 [Pubmed: 17720156]
Throughput
- Low Throughput
Curated By
- BioGRID