An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.

Publication

Endofin is required for HD-PTP and ESCRT-0 interdependent endosomal sorting of ubiquitinated transmembrane cargoes.

Kazan JM, Desrochers G, Martin CE, Jeong H, Kharitidi D, Apaja PM, Roldan A, St Denis N, Gingras AC, Lukacs GL, Pause A

Internalized and ubiquitinated signaling receptors are silenced by their intraluminal budding into multivesicular bodies aided by the endosomal sorting complexes required for transport (ESCRT) machinery. HD-PTP, an ESCRT protein, forms complexes with ESCRT-0, -I and -III proteins, and binds to Endofin, a FYVE-domain protein confined to endosomes with poorly understood roles. Using proximity biotinylation, we showed that Endofin forms a ... [more]

iScience Nov. 19, 2021; 24(11);103274 [Pubmed: 34761192]

Throughput

High Throughput

Curated By

BioGRID

Interaction Summary

ZFYVE16

Gene Ontology Biological Process

Gene Ontology Molecular Function

1-phosphatidylinositol binding [IDA]phosphatidylinositol-3,4,5-trisphosphate binding [IDA]protein binding [IPI]protein transporter activity [IMP]

Gene Ontology Cellular Component

FKBP15