Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Publication

Screening and verification of proteins that interact with HSPC238.

Tan JY, Chen JL, Huang X, Yuan CL

HSPC238 is a recently identified tumor suppressor and demonstrates ubiquitin ligase E3 enzyme activity. HSPC238 was found to be significantly downregulated in human hepatocellular carcinoma (HCC) in vivo and to inhibit the proliferation and invasion of hepatoma cells in vitro; however, the underlying molecular mechanism is largely unknown. In the present study, we screened for and identified proteins that physically ... [more]

Oncol. Rep. Dec. 01, 2015; 34(6);3097-103 [Pubmed: 26398855]

Throughput

  • Low Throughput

Curated By

  • BioGRID