PREY

VAPB

ALS8, VAMP-B, VAP-B, RP5-1018E9.1
VAMP (vesicle-associated membrane protein)-associated protein B and C
Homo sapiens

Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

MITOL regulates phosphatidic acid-binding activity of RMDN3/PTPIP51.

Ito N, Takahashi T, Shiiba I, Nagashima S, Inatome R, Yanagi S

The transfer of phospholipids from the endoplasmic reticulum (ER) to mitochondria via the mitochondria-ER contact site (MERCS) is essential for maintaining mitochondrial function and integrity. Here, we identified RMDN3/PTPIP51, possessing phosphatidic acid (PA)-transfer activity, as a neighbouring protein of the mitochondrial E3 ubiquitin ligase MITOL/MARCH5 by proximity-dependent biotin labelling using APEX2. We found that MITOL interacts with and ubiquitinates RMDN3. ... [more]

J Biochem May. 11, 2022; 171(5);529-541 [Pubmed: 34964862]

Throughput

  • Low Throughput

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
MARCH5 VAPB
Proximity Label-MS
Proximity Label-MS

An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.

High0.98BioGRID
2852846

Curated By

  • BioGRID