BAIT

NUP120

RAT2, L000003138, YKL057C
Subunit of the Nup84p subcomplex of the nuclear pore complex (NPC); contributes to nucleocytoplasmic transport and NPC biogenesis and is involved in establishment of a normal nucleocytoplasmic concentration gradient of the GTPase Gsp1p; also plays roles in several processes that may require localization of genes or chromosomes at the nuclear periphery, including double-strand break repair, transcription and chromatin silencing; homologous to human NUP160
Saccharomyces cerevisiae (S288c)
PREY

NUP159

NUP158, RAT7, FG-nucleoporin NUP159, L000002782, YIL115C
FG-nucleoporin component of central core of the nuclear pore complex; also part of the nuclear pore complex (NPC) cytoplasmic filaments; contributes directly to nucleocytoplasmic transport; regulates ADP release from the ATP-dependent RNA helicase Dbp5p; forms a stable association with Nup82p, Gle2p and two other FG-nucleoporins (Nsp1p and Nup116p)
Saccharomyces cerevisiae (S288c)

Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Maturation Kinetics of a Multiprotein Complex Revealed by Metabolic Labeling.

Onischenko E, Noor E, Fischer JS, Gillet L, Wojtynek M, Vallotton P, Weis K

All proteins interact with other cellular components to fulfill their function. While tremendous progress has been made in the identification of protein complexes, their assembly and dynamics remain difficult to characterize. Here, we present a high-throughput strategy to analyze the native assembly kinetics of protein complexes. We apply our approach to characterize the co-assembly for 320 pairs of nucleoporins (NUPs) ... [more]

Cell Dec. 23, 2019; 183(7);1785-1800.e26 [Pubmed: 33333025]

Throughput

  • High Throughput

Additional Notes

  • KARMA

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
NUP120 NUP159
Synthetic Lethality
Synthetic Lethality

A genetic interaction is inferred when mutations or deletions in separate genes, each of which alone causes a minimal phenotype, result in lethality when combined in the same cell under a given condition.

Low-BioGRID
158625

Curated By

  • BioGRID