BAIT

NUP157

L000003139, YER105C
Subunit of the inner ring of the nuclear pore complex (NPC); contributes to NPC assembly and tethering of DNA to the nuclear periphery; both Nup170p and NUP157p are similar to human Nup155p; NUP157 has a paralog, NUP170, that arose from the whole genome duplication
Saccharomyces cerevisiae (S288c)
PREY

NUP82

HRB187, linker nucleoporin NUP82, L000002941, YJL061W
Linker nucleoporin component of the nuclear pore complex (NPC); also part of the NPC cytoplasmic filaments; contributes to nucleocytoplasmic transport and NPC biogenesis; forms stable associations with three FG-nucleoporins (Nsp1p, Nup159p, and Nup116p); relocalizes to the cytosol in response to hypoxia
Saccharomyces cerevisiae (S288c)

Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Maturation Kinetics of a Multiprotein Complex Revealed by Metabolic Labeling.

Onischenko E, Noor E, Fischer JS, Gillet L, Wojtynek M, Vallotton P, Weis K

All proteins interact with other cellular components to fulfill their function. While tremendous progress has been made in the identification of protein complexes, their assembly and dynamics remain difficult to characterize. Here, we present a high-throughput strategy to analyze the native assembly kinetics of protein complexes. We apply our approach to characterize the co-assembly for 320 pairs of nucleoporins (NUPs) ... [more]

Cell Dec. 23, 2019; 183(7);1785-1800.e26 [Pubmed: 33333025]

Throughput

  • High Throughput

Additional Notes

  • KARMA

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
NUP157 NUP82
Synthetic Lethality
Synthetic Lethality

A genetic interaction is inferred when mutations or deletions in separate genes, each of which alone causes a minimal phenotype, result in lethality when combined in the same cell under a given condition.

Low-BioGRID
163574

Curated By

  • BioGRID