Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

CHD8 associates with human Staf and contributes to efficient U6 RNA polymerase III transcription.

Yuan CC, Zhao X, Florens L, Swanson SK, Washburn MP, Hernandez N

Chromatin remodeling and histone modification are essential for eukaryotic transcription regulation, but little is known about chromatin-modifying activities acting on RNA polymerase III (Pol III)-transcribed genes. The human U6 small nuclear RNA promoter, located 5' of the transcription start site, consists of a core region directing basal transcription and an activating region that recruits the transcription factors Oct-1 and Staf ... [more]

Mol. Cell. Biol. Dec. 01, 2007; 27(24);8729-38 [Pubmed: 17938208]

Throughput

  • Low Throughput

Curated By

  • BioGRID