BAIT

MEG-3

gei-12, CELE_F52D2.4, F52D2.4
gei-12 encodes a novel protein that affects embryonic viability and development of the hypodermis; interacts with GEX-3 in yeast two-hybrid assays, and is expressed in all somatic cells.
GO Process (1)
GO Function (1)
GO Component (1)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Caenorhabditis elegans
PREY

QNS-1

CELE_C24F3.4
Protein QNS-1
GO Process (0)
GO Function (0)
GO Component (0)
Caenorhabditis elegans

Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Novel LOTUS-domain proteins are organizational hubs that recruit C. elegans Vasa to germ granules.

Cipriani PG, Bay O, Zinno J, Gutwein M, Gan HH, Mayya VK, Chung G, Chen JX, Fahs H, Guan Y, Duchaine TF, Selbach M, Piano F, Gunsalus KC

We describe MIP-1 and MIP-2, novel paralogous C. elegans germ granule components that interact with the intrinsically disordered MEG-3 protein. These proteins promote P granule condensation, form granules independently of MEG-3 in the postembryonic germ line, and balance each other in regulating P granule growth and localization. MIP-1 and MIP-2 each contain two LOTUS domains and intrinsically disordered regions and ... [more]

Elife Dec. 05, 2020; 10(); [Pubmed: 34223818]

Quantitative Score

  • 0.091011779 [t_sam]

Throughput

  • High Throughput

Additional Notes

  • High confidence interactions were identified using a measure that combined fold-change of prey abundance as well as the p-value of a Student's t-test for experimental versus control purifications. Hit proteins were identified according to their p-value from the t SAM statistic as previously described (Chen et al., 2016). The t SAM statistic of the enrichment is provided in the score column.

Curated By

  • BioGRID