BAIT

DRPR

BcDNA:GH03529, CG18172, CG2086, CT41022, CT6730, Dmel\CG2086, Dmel_CG2086
draper
Drosophila melanogaster
PREY

HTS

1B1, ADD-87, Add, Add-hts, Adducin, CG34197, CG43443, CG9325, Dmel\CG43443, Dmel_CG34197, Dmel_CG9325, EST D, HTS-R1, HTS-RC, HTS_DROME, HtsF, HtsRC, Ovhts, Ovhts-RC, add-like, anon-EST:Posey9, l(2)00634, l(2)01103, l(2)k06121, l(2)k14523, Dmel_CG43443
hu li tai shao
Drosophila melanogaster

Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Publication

A protein interaction map of Drosophila melanogaster.

Giot L, Bader JS, Brouwer C, Chaudhuri A, Kuang B, Li Y, Hao YL, Ooi CE, Godwin B, Vitols E, Vijayadamodar G, Pochart P, Machineni H, Welsh M, Kong Y, Zerhusen B, Malcolm R, Varrone Z, Collis A, Minto M, Burgess S, McDaniel L, Stimpson E, Spriggs F, Williams J, Neurath K, Ioime N, Agee M, Voss E, Furtak K, Renzulli R, Aanensen N, Carrolla S, Bickelhaupt E, Lazovatsky Y, DaSilva A, Zhong J, Stanyon CA, Finley RL, White KP, Braverman M, Jarvie T, Gold S, Leach M, Knight J, Shimkets RA, McKenna MP, Chant J, Rothberg JM

Drosophila melanogaster is a proven model system for many aspects of human biology. Here we present a two-hybrid-based protein-interaction map of the fly proteome. A total of 10,623 predicted transcripts were isolated and screened against standard and normalized complementary DNA libraries to produce a draft map of 7048 proteins and 20,405 interactions. A computational method of rating two-hybrid interaction confidence ... [more]

Science Dec. 05, 2003; 302(5651);1727-36 [Pubmed: 14605208]

Throughput

  • High Throughput

Curated By

  • BioGRID