KLHL3
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
RBX1
Gene Ontology Biological Process
- Notch signaling pathway [TAS]
- SCF-dependent proteasomal ubiquitin-dependent protein catabolic process [IDA, ISS]
- cellular response to hypoxia [TAS]
- proteasome-mediated ubiquitin-dependent protein catabolic process [IDA]
- protein monoubiquitination [IDA]
- protein neddylation [IDA]
- protein ubiquitination [IDA]
- regulation of transcription from RNA polymerase II promoter in response to hypoxia [TAS]
- viral process [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
- Cul2-RING ubiquitin ligase complex [IDA]
- Cul3-RING ubiquitin ligase complex [IDA]
- Cul4A-RING E3 ubiquitin ligase complex [IDA]
- Cul4B-RING E3 ubiquitin ligase complex [IDA]
- Cul5-RING ubiquitin ligase complex [IDA]
- Cul7-RING ubiquitin ligase complex [IDA]
- SCF ubiquitin ligase complex [IDA, ISS]
- cullin-RING ubiquitin ligase complex [IDA]
- cytosol [ISS, TAS]
- nucleoplasm [TAS]
Reconstituted Complex
An interaction is detected between purified proteins in vitro.
Publication
Novel CUL3 Variant Causing Familial Hyperkalemic Hypertension Impairs Regulation and Function of Ubiquitin Ligase Activity.
Familial hyperkalemic hypertension is caused by pathogenic variants in genes of the CUL3 (cullin-3)-KLHL3 (kelch-like-family-member-3)-WNK (with no-lysine [K] kinase) pathway, manifesting clinically as hyperkalemia, metabolic acidosis, and high systolic blood pressure. The ubiquitin E3 ligase CUL3-KLHL3 targets WNK kinases for degradation to limit activation of the thiazide-sensitive NCC (Na-Cl cotransporter). All known variants in CUL3 lead to exon 9 skipping ... [more]
Throughput
- Low Throughput
Additional Notes
- The reaction contained UBA1 [E1], UBE2D3 + RBX1 [E2], and KLHL+Cul3 [E3]
- autoubiquitination of Cul3;
Related interactions
Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
---|---|---|---|---|---|---|
KLHL3 RBX1 | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - |
Curated By
- BioGRID