BAIT
PHLPP1
PHLPP, PLEKHE1, SCOP
PH domain and leucine rich repeat protein phosphatase 1
GO Process (6)
GO Function (1)
GO Component (1)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Homo sapiens
PREY
KIF5B
HEL-S-61, KINH, KNS, KNS1, UKHC
kinesin family member 5B
GO Process (10)
GO Function (5)
GO Component (6)
Gene Ontology Biological Process
- ATP catabolic process [IBA]
- axon guidance [IBA]
- cellular protein metabolic process [TAS]
- cytoskeleton-dependent intracellular transport [IBA]
- microtubule-based movement [IBA]
- positive regulation of establishment of protein localization to plasma membrane [IDA]
- positive regulation of potassium ion transport [IDA]
- regulation of membrane potential [IDA]
- stress granule disassembly [ISS]
- vesicle transport along microtubule [ISS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
Proximity Label-MS
An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.
Publication
The PHLPP1 N-Terminal Extension is a Mitotic Cdk1 Substrate and Controls an Interactome Switch.
PH domain Leucine-Rich Repeat Protein Phosphatase 1 (PHLPP1) is a tumor suppressor that directly dephosphorylates a wide array of substrates, most notably the pro-survival kinase Akt. However, little is known about the molecular mechanisms governing PHLPP1 itself. Here we report that PHLPP1 is dynamically regulated in a cell cycle-dependent manner, and deletion of PHLPP1 results in mitotic delays and increased ... [more]
Mol Cell Biol Jan. 04, 2021; (); [Pubmed: 33397691]
Throughput
- High Throughput
Additional Notes
- BioID
- Nocodazole treated cells
- PHLPP1 wildtype
- Saint express was used to identify proximity interactions and those with a BFRD of <= 1% were considered high confidence
- deltaNTE (N terminal extension of PHLPP1 deleted)
Curated By
- BioGRID