PREY

APP

AAA, ABETA, ABPP, AD1, APPI, CTFgamma, CVAP, PN-II, PN2
amyloid beta (A4) precursor protein
GO Process (29)
GO Function (8)
GO Component (21)
Homo sapiens

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

EDEM1 Regulates Amyloid Precursor Protein (APP) Metabolism and Amyloid-? Production.

Nowakowska-Golacka J, Czapiewska J, Sominka H, Sowa-Rogozinska N, Slominska-Wojewodzka M

Endoplasmic reticulum (ER) degradation-enhancing ?-mannosidase-like protein 1 (EDEM1) is a quality control factor directly involved in the endoplasmic reticulum-associated degradation (ERAD) process. It recognizes terminally misfolded proteins and directs them to retrotranslocation which is followed by proteasomal degradation in the cytosol. The amyloid-? precursor protein (APP) is synthesized and N-glycosylated in the ER and transported to the Golgi for maturation ... [more]

Int J Mol Sci Dec. 23, 2021; 23(1); [Pubmed: 35008544]

Throughput

  • Low Throughput

Curated By

  • BioGRID