BAIT

NHEJ1

XLF

nonhomologous end-joining factor 1

Fanconi Anemia Project

GO Process (6)

GO Function (1)

GO Component (2)

Gene Ontology Biological Process

B cell differentiation [IMP]

T cell differentiation [IMP]

central nervous system development [NAS]

double-strand break repair via nonhomologous end joining [IMP]

positive regulation of ligase activity [NAS]

response to ionizing radiation [IDA]

Gene Ontology Molecular Function

protein binding [IPI]

Gene Ontology Cellular Component

nonhomologous end joining complex [IDA]

CRISPR Database HGNC Alliance of Genome Resources OMIM Entrez Gene RefSeq UniprotKB Ensembl

Homo sapiens

PREY

PRKACB

PKA C-beta, PKACB, RP11-82H13.1

protein kinase, cAMP-dependent, catalytic, beta

Alzheimer's Disease Project

Human Papilloma Virus (HPV) Project

GO Process (22)

GO Function (5)

GO Component (5)

Gene Ontology Biological Process

activation of phospholipase C activity [TAS]

activation of protein kinase A activity [TAS]

adenylate cyclase-modulating G-protein coupled receptor signaling pathway [TAS]

blood coagulation [TAS]

carbohydrate metabolic process [TAS]

cellular response to glucagon stimulus [TAS]

energy reserve metabolic process [TAS]

epidermal growth factor receptor signaling pathway [TAS]

fibroblast growth factor receptor signaling pathway [TAS]

gluconeogenesis [TAS]

glucose metabolic process [TAS]

innate immune response [TAS]

intracellular signal transduction [TAS]

neurotrophin TRK receptor signaling pathway [TAS]

protein phosphorylation [IDA]

regulation of insulin secretion [TAS]

signal transduction [TAS]

small molecule metabolic process [TAS]

synaptic transmission [TAS]

transmembrane transport [TAS]

triglyceride catabolic process [TAS]

water transport [TAS]

Gene Ontology Molecular Function

ATP binding [IDA]
cAMP-dependent protein kinase activity [IDA]
magnesium ion binding [IDA]
protein binding [IPI]
ubiquitin protein ligase binding [IDA]

Gene Ontology Cellular Component

cAMP-dependent protein kinase complex [TAS]

centrosome [IDA]

extracellular vesicular exosome [IDA]

nucleoplasm [TAS]

CRISPR Database HGNC Alliance of Genome Resources OMIM Entrez Gene RefSeq UniprotKB Ensembl

Homo sapiens

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

The conserved Tpk1 regulates non-homologous end joining double-strand break repair by phosphorylation of Nej1, a homolog of the human XLF.

Jessulat M, Amin S, Hooshyar M, Malty R, Moutaoufik MT, Zilocchi M, Istace Z, Phanse S, Aoki H, Omidi K, Burnside D, Samanfar B, Aly KA, Golshani A, Babu M

The yeast cyclic AMP-dependent protein kinase A (PKA) is a ubiquitous serine-threonine kinase, encompassing three catalytic (Tpk1-3) and one regulatory (Bcy1) subunits. Evidence suggests PKA involvement in DNA damage checkpoint response, but how DNA repair pathways are regulated by PKA subunits remains inconclusive. Here, we report that deleting the tpk1 catalytic subunit reduces non-homologous end joining (NHEJ) efficiency, whereas tpk2-3 ... [more]

Nucleic Acids Res Dec. 20, 2020; 49(14);8145-8160 [Pubmed: 34244791]

Throughput

Low Throughput

Curated By

BioGRID