An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

NUMB facilitates autophagy initiation through targeting SCF?-TrCP2 complex.

Li H, Shu S, Zhou M, Chen Y, Xiao A, Ma Y, Zhu F, Hu Z, Nie J

Given the critical role of SCF E3 ligases in autophagy by modulating the protein stability of various autophagic components, the activity of SCF should be tightly controlled to maintain the autophagic flux. We here showed that Numb, a multifunctional adaptor protein, increased the protein abundance of DEPTOR, which is an inhibitor of mTORC1, leading to increased autophagy flux. In vitro ... [more]

Cell Death Differ Jan. 11, 2022; (); [Pubmed: 35017646]

Throughput

Low Throughput

Curated By

BioGRID

Interaction Summary

NUMB

Gene Ontology Biological Process

Gene Ontology Molecular Function

protein binding [IPI]

Gene Ontology Cellular Component

DEPTOR