An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Targeted substrate loop insertion by VCP/p97 during PP1 complex disassembly.

van den Boom J, Kueck AF, Kravic B, Mueschenborn H, Giesing M, Pan D, Kaschani F, Kaiser M, Musacchio A, Meyer H

The AAA-ATPase VCP/p97/Cdc48 unfolds proteins by threading them through its central pore, but how substrates are recognized and inserted into the pore in diverse pathways has remained controversial. Here, we show that p97, with its adapter p37, binds an internal recognition site (IRS) within inhibitor-3 (I3) and then threads a peptide loop into its channel to strip I3 off protein ... [more]

Nat Struct Mol Biol Dec. 01, 2020; 28(12);964-971 [Pubmed: 34824462]

Throughput

Low Throughput

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
PPP1R7 PPP1R11	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Boldt K (2016)	High	-	BioGRID	-

Curated By

BioGRID

Interaction Summary

PPP1R11

Gene Ontology Molecular Function

protein binding [IPI]

Gene Ontology Cellular Component

PPP1R7

Gene Ontology Biological Process