PREY

UBXN2B

3110003A22Rik, 6430407D20Rik, AI451665, p37, RP23-340E12.2
UBX domain protein 2B
Mus musculus

Reconstituted Complex

An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.

Publication

Targeted substrate loop insertion by VCP/p97 during PP1 complex disassembly.

van den Boom J, Kueck AF, Kravic B, Mueschenborn H, Giesing M, Pan D, Kaschani F, Kaiser M, Musacchio A, Meyer H

The AAA-ATPase VCP/p97/Cdc48 unfolds proteins by threading them through its central pore, but how substrates are recognized and inserted into the pore in diverse pathways has remained controversial. Here, we show that p97, with its adapter p37, binds an internal recognition site (IRS) within inhibitor-3 (I3) and then threads a peptide loop into its channel to strip I3 off protein ... [more]

Nat Struct Mol Biol Dec. 01, 2020; 28(12);964-971 [Pubmed: 34824462]

Throughput

  • Low Throughput

Curated By

  • BioGRID