An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.

Publication

Comparative Application of BioID and TurboID for Protein-Proximity Biotinylation.

May DG, Scott KL, Campos AR, Roux KJ

BioID is a well-established method for identifying protein-protein interactions and has been utilized within live cells and several animal models. However, the conventional labeling period requires 15-18 h for robust biotinylation which may not be ideal for some applications. Recently, two new ligases termed TurboID and miniTurbo were developed using directed evolution of the BioID ligase and were able to ... [more]

Cells Dec. 25, 2019; 9(5); [Pubmed: 32344865]

Throughput

High Throughput

Additional Notes

FDR for protein identification was set to 1%
TurboID

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
LMNA SEC22B	Proximity Label-MS Proximity Label-MS An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.	Go CD (2021)	High	11.43	BioGRID	2994604
LMNA SEC22B	Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.	Dittmer TA (2014)	High	-	BioGRID	-

Curated By

BioGRID

Interaction Summary

LMNA

Gene Ontology Biological Process

Gene Ontology Molecular Function

protein binding [IPI]structural molecule activity [TAS]

Gene Ontology Cellular Component

SEC22B