BAIT

LMNA

CDCD1, CDDC, CMD1A, CMT2B1, EMD2, FPL, FPLD, FPLD2, HGPS, IDC, LDP1, LFP, LGMD1B, LMN1, LMNC, LMNL1, PRO1, RP11-54H19.1
lamin A/C
Cell Cycle - Senescence Project
Homo sapiens
PREY

TEX264

ZSIG11, UNQ337/PRO536
testis expressed 264
Autophagy Project
Fanconi Anemia Project
GO Process (0)
GO Function (0)
GO Component (1)

Gene Ontology Cellular Component

Homo sapiens

Proximity Label-MS

An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.

Publication

Comparative Application of BioID and TurboID for Protein-Proximity Biotinylation.

May DG, Scott KL, Campos AR, Roux KJ

BioID is a well-established method for identifying protein-protein interactions and has been utilized within live cells and several animal models. However, the conventional labeling period requires 15-18 h for robust biotinylation which may not be ideal for some applications. Recently, two new ligases termed TurboID and miniTurbo were developed using directed evolution of the BioID ligase and were able to ... [more]

Cells Dec. 25, 2019; 9(5); [Pubmed: 32344865]

Throughput

  • High Throughput

Additional Notes

  • FDR for protein identification was set to 1%
  • TurboID

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
LMNA TEX264
Proximity Label-MS
Proximity Label-MS

An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.

High50BioGRID
2994616

Curated By

  • BioGRID