An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.

Publication

Ubiquitin ligase MARCH5 localizes to peroxisomes to regulate pexophagy.

Zheng J, Chen X, Liu Q, Zhong G, Zhuang M

Mitochondria and peroxisomes are independent but functionally closely related organelles. A few proteins have been characterized as dual-organelle locating proteins with distinct or similar roles on mitochondria and peroxisomes. MARCH5 is a mitochondria-associated ubiquitin ligase best known for its regulatory role in mitochondria quality control, fission, and fusion. Here, we used a proximity tagging system, PUP-IT, and identified new interacting ... [more]

J Cell Biol Dec. 03, 2021; 221(1); [Pubmed: 34747980]

Throughput

High Throughput|Low Throughput

Additional Notes

LTP hits identified by western

Curated By

BioGRID

Interaction Summary

MARCH5

Gene Ontology Biological Process

Gene Ontology Molecular Function

GTPase binding [IPI]protein binding [IPI]ubiquitin-protein transferase activity [IDA, IMP]

Gene Ontology Cellular Component

CYB5R1