An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Circular RNA circRNF13 inhibits proliferation and metastasis of nasopharyngeal carcinoma via SUMO2.

Mo Y, Wang Y, Zhang S, Xiong F, Yan Q, Jiang X, Deng X, Wang Y, Fan C, Tang L, Zhang S, Gong Z, Wang F, Liao Q, Guo C, Li Y, Li X, Li G, Zeng Z, Xiong W

Circular RNAs (circRNAs) are widely expressed in human cells and are closely associated with cancer development. However, they have rarely been investigated in the context of nasopharyngeal carcinoma (NPC).We screened a new circRNA, circRNF13, in NPC cells using next-generation sequencing of mRNA. Reverse transcription polymerase chain reaction and RNA fluorescence in situ hybridization were used to detect circRNF13 expression in ... [more]

Mol Cancer Dec. 31, 2020; 20(1);112 [Pubmed: 34465340]

Throughput

Low Throughput

Curated By

BioGRID

Interaction Summary

SLC2A1

Gene Ontology Biological Process

Gene Ontology Molecular Function

glucose transmembrane transporter activity [IDA]identical protein binding [IPI]protein binding [IPI]protein self-association [IDA]

Gene Ontology Cellular Component

UBE2I