SQSTM1
Gene Ontology Biological Process
- apoptotic signaling pathway [TAS]
- autophagy [IMP, TAS]
- endosomal transport [TAS]
- intracellular signal transduction [TAS]
- macroautophagy [ISS]
- negative regulation of apoptotic process [TAS]
- neurotrophin TRK receptor signaling pathway [TAS]
- positive regulation of apoptotic process [TAS]
- positive regulation of macroautophagy [IMP]
- positive regulation of transcription from RNA polymerase II promoter [TAS]
- protein localization [TAS]
- protein phosphorylation [NAS]
- regulation of I-kappaB kinase/NF-kappaB signaling [IMP]
- regulation of Ras protein signal transduction [NAS]
- response to stress [TAS]
- ubiquitin-dependent protein catabolic process [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
VAPB
Gene Ontology Biological Process
- COPII-coated vesicle budding [IMP]
- ER to Golgi vesicle-mediated transport [IMP]
- activation of signaling protein activity involved in unfolded protein response [IDA]
- cellular calcium ion homeostasis [IMP]
- endoplasmic reticulum organization [IMP]
- endoplasmic reticulum unfolded protein response [IMP]
- modulation by virus of host morphology or physiology [IDA]
- negative regulation by host of viral genome replication [IDA]
- negative regulation by host of viral release from host cell [IDA]
- negative regulation by virus of viral protein levels in host cell [IDA]
- positive regulation by host of viral genome replication [IDA]
- positive regulation by host of viral release from host cell [IDA]
- positive regulation of viral genome replication [IMP]
- small molecule metabolic process [TAS]
- sphingolipid biosynthetic process [TAS]
- sphingolipid metabolic process [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
Sequestosome 1 Is Part of the Interaction Network of VAPB.
VAPB (Vesicle-Associated-membrane Protein-associated protein B) is a tail-anchored membrane protein of the endoplasmic reticulum that can also be detected at the inner nuclear membrane. As a component of many contact sites between the endoplasmic reticulum and other organelles, VAPB is engaged in multiple protein interactions with a plethora of binding partners. A mutant version of VAPB, P56S-VAPB, which results from ... [more]
Throughput
- Low Throughput
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| VAPB SQSTM1 | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | Low | - | BioGRID | 3391586 | |
| VAPB SQSTM1 | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - | |
| VAPB SQSTM1 | Co-localization Co-localization Interaction inferred from two proteins that co-localize in the cell by indirect immunofluorescence only when in addition, if one gene is deleted, the other protein becomes mis-localized. Also includes co-dependent association of proteins with promoter DNA in chromatin immunoprecipitation experiments. | Low | - | BioGRID | 3391585 | |
| SQSTM1 VAPB | Proximity Label-MS Proximity Label-MS An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods. | High | - | BioGRID | 3306837 | |
| SQSTM1 VAPB | Reconstituted Complex Reconstituted Complex An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator. | Low | - | BioGRID | - |
Curated By
- BioGRID