An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Sequestosome 1 Is Part of the Interaction Network of VAPB.

James C, Lenz C, Urlaub H, Kehlenbach RH

VAPB (Vesicle-Associated-membrane Protein-associated protein B) is a tail-anchored membrane protein of the endoplasmic reticulum that can also be detected at the inner nuclear membrane. As a component of many contact sites between the endoplasmic reticulum and other organelles, VAPB is engaged in multiple protein interactions with a plethora of binding partners. A mutant version of VAPB, P56S-VAPB, which results from ... [more]

Int J Mol Sci Dec. 09, 2021; 22(24); [Pubmed: 34948065]

Throughput

Low Throughput

Curated By

BioGRID

Interaction Summary

VAPB

Gene Ontology Biological Process

Gene Ontology Molecular Function

FFAT motif binding [IMP]beta-tubulin binding [IDA]enzyme binding [IPI]microtubule binding [IDA]protein binding [IPI]protein heterodimerization activity [IPI]protein homodimerization activity [IPI]

Gene Ontology Cellular Component

TMEM43