BAIT

IBTK

BTBD26, BTKI, RP1-93K22.1
inhibitor of Bruton agammaglobulinemia tyrosine kinase
Homo sapiens
PREY

GSK3B

glycogen synthase kinase 3 beta
GO Process (35)
GO Function (11)
GO Component (7)

Gene Ontology Biological Process

Homo sapiens

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

IBtk? Activates the ?-Catenin-Dependent Transcription of MYC through Ubiquitylation and Proteasomal Degradation of GSK3? in Cancerous B Cells.

Vecchio E, Nistico N, Golino G, Iaccino E, Maisano D, Mimmi S, Aloisio A, Renna M, Avagliano A, Arcucci A, Fiume G, Quinto I

The IBTK gene encodes the IBtk? protein that is a substrate receptor of E3 ubiquitin ligase, Cullin 3. We have previously reported the pro-tumorigenic activity of Ibtk in MYC-dependent B-lymphomagenesis observed in E?-myc transgenic mice. Here, we provide mechanistic evidence of the functional interplay between IBtk? and MYC. We show that IBtk?, albeit indirectly, activates the ?-catenin-dependent transcription of the ... [more]

Int J Mol Sci Feb. 12, 2022; 23(4); [Pubmed: 35216159]

Throughput

  • Low Throughput

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
GSK3B IBTK
Proximity Label-MS
Proximity Label-MS

An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.

High-BioGRID
3537884

Curated By

  • BioGRID