BAIT

PPP1R13L

IASPP, NKIP1, RAI, RAI4
protein phosphatase 1, regulatory subunit 13 like
GO Process (0)
GO Function (3)
GO Component (3)
Homo sapiens

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

iASPP is essential for HIF-1? stabilization to promote angiogenesis and glycolysis via attenuating VHL-mediated protein degradation.

Zhao D, Zheng S, Wang X, Liu H, Zhao K, Li L, Hu Y

Hypoxia-inducible factor-1? (HIF-1?) plays central roles in the hypoxia response. It is highly expressed in multiple cancers, but not always correlated with hypoxia. Mutation of the von Hippel-Lindau (VHL) gene, which encodes an E3 ligase, contributes to the constructive activation of HIF-1? in specific tumor types, as exemplified by renal cell carcinoma; but how VHL wild-type tumors acquire this ability ... [more]

Oncogene Dec. 01, 2021; 41(13);1944-1958 [Pubmed: 35169254]

Throughput

  • Low Throughput

Curated By

  • BioGRID