BAIT
NUP43
bA350J20.1, p42
nucleoporin 43kDa
GO Process (10)
GO Function (1)
GO Component (4)
Gene Ontology Biological Process
- carbohydrate metabolic process [TAS]
- cytokine-mediated signaling pathway [TAS]
- glucose transport [TAS]
- hexose transport [TAS]
- mitotic cell cycle [TAS]
- mitotic nuclear envelope disassembly [TAS]
- regulation of glucose transport [TAS]
- small molecule metabolic process [TAS]
- transmembrane transport [TAS]
- viral process [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
PREY
ATP8A1
ATPASEII, ATPIA, ATPP2
ATPase, aminophospholipid transporter (APLT), class I, type 8A, member 1
GO Process (4)
GO Function (2)
GO Component (5)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
Proximity Label-MS
An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.
Publication
An improved smaller biotin ligase for BioID proximity labeling.
The BioID method uses a promiscuous biotin ligase to detect protein-protein associations as well as proximate proteins in living cells. Here we report improvements to the BioID method centered on BioID2, a substantially smaller promiscuous biotin ligase. BioID2 enables more-selective targeting of fusion proteins, requires less biotin supplementation, and exhibits enhanced labeling of proximate proteins. Thus BioID2 improves the efficiency ... [more]
Mol Biol Cell Apr. 15, 2016; 27(8);1188-96 [Pubmed: 26912792]
Throughput
- High Throughput
Additional Notes
- Proteins identified using BioID2
Curated By
- BioGRID